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  • Why are there two Incubation ranges for SCD?

  • Two incubation temperature ranges are specified by the USP: 20–25°C for SCD and 30–35°C for FTM. The 30–35°C range is used because this approximates human body temperature and mesophilic organisms will grow. Many potential microbial contaminants would be of human origin such as the micrococci and staphylococci of skin flora.

  • How often should you check the temperature of an incubator?

  • The incubation temperature is a key parameter for the determination of microbial strains. Therefore, its proper functioning is of paramount importance. The incubator’s temperature should be checked and recorded every morning at the end of the overnight incubation.

  • How long is the incubation period for aseptically filled products?

  • The incubation period for aseptically filled (nonterminally sterilized) products is not less than (NLT) 14 days and for terminally sterilized products is NLT 7 days, if the membrane filtration method is used.3 The incubation temperature is a key parameter for the determination of microbial strains.

  • What should the incubation temperature be in a forest?

  • In forest openings, grasslands, and deserts, insolation may elevate surface soil temperatures. Isolation protocols often call for incubation at room temperature or 25°C, and at these temperatures most colonies develop within 2–10 days. Prevailing temperatures in temperate forest soils, however, are generally much lower (Carreiro and Koske 1992).

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